Porcine factor VIII:C prepared by affinity interaction with von Willebrand factor and heterologous antibodies: sodium dodecyl sulfate polyacrylamide gel analysis.

A procedure was developed to concentrate and partially purify porcine factor VIll:C from plasma by using conventional precipitation and chromatographic techniques. Blood from heparinized animal(s) was collected in citrate. soybean trypsin inhibitor. #{128}-aminocaproic acid and benzamidine. After aluminum hydroxide adsorption. polyethylene glycol-6000 precipatation. QAE-cellulose chromatography. and dextran sulfate-agarose chromatography. the factor VlIl:C concentrate was 8.iOO-fold purified with an overall yield of 24%. and thrombin treatment of the factor VIII:C gave an activation coefficient of up to 56. The activation coefficient of plasma factor VIII:C averaged 35. Affinity chromatography of the factor Vlll:C concentrate on von Willebrand factor-agarose produced a two fold increase in specific activity. This product was applied to a second affinity resin. the acidic IgG fraction of human antihuman factor VllI:C coupled to agarose. The inactive